Measurement of IgE to purified sesame storage proteins in sera from sesame allergic Japanese children

Methods The three storage proteins were all purified from unpolished sesame seeds extracted at pH 7,5. The 11S globulin and the 7S vicilin were enriched by precipitation in water. Pure 11S globulin was obtained by a further precipitation with 35% ammonium sulfate followed by anion exchange chromatography. Pure 7S vicilin was obtained by adding gel filtration and anion exchange chromatography. The 2S albumin was purified by cation exchange chromatography and gel filtration. Identities were confirmed by MALDI-TOF analysis. The three purified storage proteins were conjugated to the ImmunoCAP matrix and quantitative IgE responses in sera from five sesame allergic Japanese children were measured.